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dulbecco’s modified eagle medium ham’s f-12 50/50 mix (dmem/f12 50/50)  (Corning Life Sciences)

 
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    Corning Life Sciences dulbecco’s modified eagle medium ham’s f-12 50/50 mix (dmem/f12 50/50)
    Dulbecco’s Modified Eagle Medium Ham’s F 12 50/50 Mix (Dmem/F12 50/50), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+ham%E2%80%99s+f-12/rpmi+1640/pm40651227-52-14-24
    Average 90 stars, based on 1 article reviews
    dulbecco’s modified eagle medium ham’s f-12 50/50 mix (dmem/f12 50/50) - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Metabolic programs drive function of therapeutic NK cells in hypoxic tumor environments
    Article Snippet: After thaw, freezing media was removed by centrifugation, and cells were cultured with IL-15 (1 ng/ml; R&D Systems) in B0 media: 60% DMEM (Corning, catalog no. 10-017-CV), 30% modified Ham’s F-12 (Corning, catalog no. 10-080-CV), 10% heat-inactivated human AB serum (Valley Biomedical Inc., catalog no. HP1022), penicillin/streptomycin (100 U/ml; Gibco, catalog no. 15140-122), 20 μM β2-mercaptoethanol (Sigma-Aldrich, catalog no. M7522), 50 μM ethanolamine (Sigma-Aldrich, catalog no. E0135), ascorbic acid (20 μg/ml; Sigma-Aldrich, catalog no. A4544), and sodium selenite (5 ng/ml; Sigma-Aldrich, catalog no. S5261) for up to 7 days with a media change on day 4.

    Article Title: Metabolic programs drive function of therapeutic NK cells in hypoxic tumor environments.
    Article Snippet: After thaw, freezing media was removed by centrifugation, and cells were cultured with IL- 15 (1 ng/ml; R&D Systems) in B0 media: 60% DMEM (Corning, catalog no. 10- 017- CV), 30% modified Ham’s F- 12 (Corning, catalog no. 10- 080- CV), 10% heat- inactivated human AB serum (Valley Biomedical Inc., catalog no. HP1022), penicillin/streptomycin (100 U/ml; Gibco, catalog no. 15140- 122), 20 μM β2- mercaptoethanol (SigmaAldrich, catalog no. M7522), 50 μM ethanolamine (Sigma- Aldrich, catalog no. E0135), ascorbic acid (20 μg/ml; Sigma- Aldrich, catalog no. A4544), and sodium selenite (5 ng/ml; Sigma- Aldrich, catalog no. S5261) for up to 7 days with a media change on day 4.

    Cell Culture:

    Article Title: Metabolic programs drive function of therapeutic NK cells in hypoxic tumor environments
    Article Snippet: After thaw, freezing media was removed by centrifugation, and cells were cultured with IL-15 (1 ng/ml; R&D Systems) in B0 media: 60% DMEM (Corning, catalog no. 10-017-CV), 30% modified Ham’s F-12 (Corning, catalog no. 10-080-CV), 10% heat-inactivated human AB serum (Valley Biomedical Inc., catalog no. HP1022), penicillin/streptomycin (100 U/ml; Gibco, catalog no. 15140-122), 20 μM β2-mercaptoethanol (Sigma-Aldrich, catalog no. M7522), 50 μM ethanolamine (Sigma-Aldrich, catalog no. E0135), ascorbic acid (20 μg/ml; Sigma-Aldrich, catalog no. A4544), and sodium selenite (5 ng/ml; Sigma-Aldrich, catalog no. S5261) for up to 7 days with a media change on day 4.

    Article Title: Metabolic programs drive function of therapeutic NK cells in hypoxic tumor environments.
    Article Snippet: After thaw, freezing media was removed by centrifugation, and cells were cultured with IL- 15 (1 ng/ml; R&D Systems) in B0 media: 60% DMEM (Corning, catalog no. 10- 017- CV), 30% modified Ham’s F- 12 (Corning, catalog no. 10- 080- CV), 10% heat- inactivated human AB serum (Valley Biomedical Inc., catalog no. HP1022), penicillin/streptomycin (100 U/ml; Gibco, catalog no. 15140- 122), 20 μM β2- mercaptoethanol (SigmaAldrich, catalog no. M7522), 50 μM ethanolamine (Sigma- Aldrich, catalog no. E0135), ascorbic acid (20 μg/ml; Sigma- Aldrich, catalog no. A4544), and sodium selenite (5 ng/ml; Sigma- Aldrich, catalog no. S5261) for up to 7 days with a media change on day 4.

    Modification:

    Article Title: Metabolic programs drive function of therapeutic NK cells in hypoxic tumor environments
    Article Snippet: After thaw, freezing media was removed by centrifugation, and cells were cultured with IL-15 (1 ng/ml; R&D Systems) in B0 media: 60% DMEM (Corning, catalog no. 10-017-CV), 30% modified Ham’s F-12 (Corning, catalog no. 10-080-CV), 10% heat-inactivated human AB serum (Valley Biomedical Inc., catalog no. HP1022), penicillin/streptomycin (100 U/ml; Gibco, catalog no. 15140-122), 20 μM β2-mercaptoethanol (Sigma-Aldrich, catalog no. M7522), 50 μM ethanolamine (Sigma-Aldrich, catalog no. E0135), ascorbic acid (20 μg/ml; Sigma-Aldrich, catalog no. A4544), and sodium selenite (5 ng/ml; Sigma-Aldrich, catalog no. S5261) for up to 7 days with a media change on day 4.

    Article Title: Metabolic programs drive function of therapeutic NK cells in hypoxic tumor environments.
    Article Snippet: After thaw, freezing media was removed by centrifugation, and cells were cultured with IL- 15 (1 ng/ml; R&D Systems) in B0 media: 60% DMEM (Corning, catalog no. 10- 017- CV), 30% modified Ham’s F- 12 (Corning, catalog no. 10- 080- CV), 10% heat- inactivated human AB serum (Valley Biomedical Inc., catalog no. HP1022), penicillin/streptomycin (100 U/ml; Gibco, catalog no. 15140- 122), 20 μM β2- mercaptoethanol (SigmaAldrich, catalog no. M7522), 50 μM ethanolamine (Sigma- Aldrich, catalog no. E0135), ascorbic acid (20 μg/ml; Sigma- Aldrich, catalog no. A4544), and sodium selenite (5 ng/ml; Sigma- Aldrich, catalog no. S5261) for up to 7 days with a media change on day 4.



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol to study ductal progenitor-like cells from the adult human pancreas using 3D suspension and methylcellulose-based culture systems

    doi: 10.1016/j.xpro.2025.103847

    Figure Lengend Snippet: Colony assay medium

    Article Snippet: DMEM/F12/PS: Dulbecco’s Modified Eagle’s Medium/Hams F-12 (DMEM/F12, 10-092-CV, Corning) and 1x PS (15140-122, Gibco).

    Techniques: Colony Assay, Concentration Assay, Suspension